Podoplanin

Podoplanin is a transmembrane protein expressed by lymphatic endothelial cells, fibroblasts, osteocytes,
follicular dendritic cells, smooth and striated muscle cells, myoepithelial cells, Cajal cells, squamous epithelial basal cells, prostatic epithelial basal cells, immature Sertoli cells, gastric crypt cells, renal glomerular podocytes, and Schwann cells. In tumors, podoplanin is detected in follicular dendritic cell tumors, dermatofibromas, lymphangiomas, Kaposi’s sarcoma, hemangioblastomas, seminomas, epithelioid mesotheliomas, and a subset of angiosarcomas with lymphatic differentiation.

Intended use

Podoplanin is useful for differentiating between malignant mesotheliomas and adenocarcinomas, identifying seminomas, and visualizing lymphatic vessels and lymphatic invasion of primary tumors when used with a panel of antibodies.

Specifications
product code: 8515-C010	Clone: D2-40 [Mouse]
Staining pattern: Cytoplasmic staining	Control tissue: Tonsil, appendix
Associated panels: Dermatopathology
Assessment criteria
A strong, distinct predominantly cytoplasmic staining reaction of virtually all lymphatic endothelial cells and the follicular dendritic cells in the germinal centre of tonsil
An at least moderate, distinct predominantly cytoplasmic staining reaction of the Schwann cells of the muscularis propria in the appendix
No staining reaction should be seen in endothelial cells of the blood vessels, epithelial cells and smooth muscle cells in appendix