BOB.1

BOB.1, B-cell Octamer Binding Protein-1, is a B-cell specific coactivator and interacts with OCT.1 and
OCT.2 transcription factors. It mediates antigendependent germinal center formation. The expression
of BOB.1 is largely to mature B-cells in germinal center, and also in mantle zone and plasma cells

Intended use

Anti-BOB.1 antibody is useful for identification of B-cells in germinal centers and mantle cells, and for differentiation of lymphoma, especially for identification of the lineage of CD20 negative B-cell neoplasms and differentiation of classical Hodgkin’s lymphoma (BOB.1 negative) from primary mediastinal large B-cell lymphoma (BOB.1 positive) when used in a panel with other antibodies. The strong nuclear expression of BOB.1 and OCT.2 by germinal center derived lymphomas makes these antibodies a novel class of road-spectrum B-lineage IHC markers to aid in the differential diagnosis of lymphomas.

Specifications
product code: 8462-C010	Clone: ZM74 [Mouse]
Staining pattern: Nuclear/cytoplasmic staining	Control tissue: Appendix, tonsil
Assessment criteria
A moderate staining reaction of lymphocytes in lamina propria of appendix
A weak to moderate staining reaction in all germinal centre B-cells in tonsil
An at least weak staining reaction of B-cells in the mantle zone of tonsil