TRPS1

TRPS1 (trichorhinophalangeal syndrome type 1) belongs to the GATA3 family of zinc finger transcription factors and plays a critical role in the development of cartilage, bone, kidney, breast, and hair
follicle. TRPS1 is highly expressed in luminal epithelial cells of the breast, as well as in both epithelial and stromal cells of the uterine cervix. It is also found in neuronal cells and scattered lymphocytes within the gastrointestinal tract (appendix/colon). TRPS1 expression is also observed in suprabasal cells of non-keratinizing squamous epithelium and epithelial cells of the hair follicle, sebocytes, and eccrine glands of the skin. Weak to moderate TRPS1 expression has also been detected in epithelial cells of the salivary gland, gallbladder, fallopian tube, lung, renal tubules, thyroid gland, and parathyroid gland, as well as in spermatogonia of the testis and smooth muscle cells. Occasional TRPS1 expression has been reported in stromal fibroblasts within normal tissue specimens. TRPS1 is absent in appendiceal epithelium and myoepithelial cells surrounding breast glands.

Intended use

TRPS1 has a higher sensitivity compared to established markers such as GATA3, GCDFP-15, Mammaglobin, and FOXC1, TRPS1 has recently gained recognition as a valuable adjunct IHC biomarker for diagnosing breast carcinoma. This is especially important in triple-negative breast cancers (TNBCs), where conventional markers
often show low or absent expression. The frequent retention of TRPS1 expression in TNBCs makes it particularly useful for diagnosing primary tumors as well as identifying metastatic breast carcinoma.

Specifications
product code: 8359-C010	Clone: ZR382 [Rabbit monoclonal]
Staining pattern: Nuclear staining	Control tissue:  Breast hyperplasia, uterine cervix, appendix
Associated panels: Breast
Assessment criteria
Breast Hyperplasia: Luminal epithelial cells should show a strong nuclear staining reaction.
Uterine cervix: Most suprabasal squamous epithelial cells and dispersed stromal cells should display an at least weak to moderate nuclear staining reaction.
Appendix: No staining reaction in epithelial cells.
literature:
Lennartz M, Löhr N, Höflmayer D, Dwertmann Rico S, von Bargen C, Kind S, Reiswich V, Viehweger F, Lutz F, Bertram V, Fraune C, Gorbokon N, Weidemann S, Blessin NC, Hube-Magg C, Menz A, Schlichter R, Krech T, Hinsch A, Burandt E, Sauter G, Simon R, Kluth M, Marx AH, Lebok P, Dum D, Minner S, Jacobsen F, Clauditz TS, Bernreuther C, Steurer S. TRPS1 is a Highly Sensitive Marker for Breast Cancer: A Tissue Microarray Study Evaluating More Than 19,000 Tumors From 152 Different Tumor Entities. Am J Surg Pathol. 2024; 48(6): 637-651. https://doi.org/10.1097/pas.0000000000002213.
Bachert SE, Di J, Zhang S, Short HE, Piecoro DW, McDonald RJ, Myint ZW, Hensley PJ, Allison DB. TRPS1 expression in primary and metastatic prostatic adenocarcinoma, muscle invasive bladder urothelial carcinoma, and breast carcinoma: Is TRPS1 truly specific and sensitive for a breast primary? Hum Pathol. 2024; 143: 42-49. https://doi.org/10.1016/j.humpath.2023.11.012.
Ai D, Yao J, Yang F, Huo L, Chen H, Lu W, Soto LMS, Jiang M, Raso MG, Wang S, Bell D, Liu J, Wang H, Tan D, Torres-Cabala C, Gan Q, Wu Y, Albarracin C, Hung MC, Meric-Bernstam F, Wistuba, II, Prieto VG, Sahin AA, Ding Q. TRPS1: a highly sensitive and specific marker for breast carcinoma, especially for triple-negative breast cancer. Mod Pathol. 2021; 34(4): 710-719. https://doi.org/10.1038/s41379-020-00692-8
Hashmi AA, Brogi E, Wen HY. Trichorhinophalangeal syndrome type 1 (TRPS1) in breast pathology: diagnostic utility and pitfalls. Diagn Pathol. 2025; 20(1): 26. https://doi.org/10.1186/s13000-025-01623-4.
Yoon EC, Wang G, Parkinson B, Huo L, Peng Y, Wang J, Salisbury T, Wu Y, Chen H, Albarracin CT, Resetkova E, Middleton LP, Krishnamurthy S, Gan Q, Sun H, Huang X, Shen T, Chen W, Parwani AV, Sahin AA, Li Z, Ding Q. TRPS1, GATA3, and SOX10 expression in triple-negative breast carcinoma. Hum Pathol. 2022; 125: 97-107. https://doi.org/10.1016/j.humpath.2022.04.006.