WT1

Wilms’ tumor-1 protein (WT1) is a transcription factor that plays a role in the development of genitourinary organs and is involved in the induction of Wilms’ tumor (nephroblastoma), a pediatric renal malignancy. WT1 proteins are synthesized and reside in the cytoplasm in an inactive form. When activated, they are translocated to the nucleus. In normal epithelia, nuclear WT1 protein expression is largely restricted to the surface epithelium and inclusion cysts of ovaries and fallopian tubes, and is absent in endometrial and cervical epithelia.

Intended use

Tissue-Tek Genie anti-WT1 Rabbit Monoclonal Antibody [EP122] is useful for identifying Wilms’ tumor, distinguishing malignant mesothelioma from lung adenocarcinoma, and distinguishing ovarian serous carcinoma from non-serous carcinoma when used with a panel of antibodies

Specifications
product code: 8340-C010	Clone: EP122 [Rabbit]
Staining pattern: Nuclear staining	Control tissue: Fallobian tube, kidney
Associated panels: Carcinoma - general panel
Assessment criteria
A strong, distinct nuclear staining in virtually all epithelial and smooth muscle cells of the fallopian tube
An at least moderate, nuclear staining of virtually all podocytes and parietal epithelial cells of the Bowman’s capsule in kidney
No staining of epithelial cells of the tubules in Kidney
* A cytoplasmic staining reaction in a variety of cells (like endothelia, smooth muscle, striated muscle and plasma cells) can be expected