DUO Melan A / Ki67

Melan-A, also known as Melanoma Antigen Recognized by T-cell 1 (MART-1), is a protein associated with endoplasmic reticulum and melanosomes and expressed in melanocytes. Melan A is expressed in all normal melanocytes and is detected in 80-100% of melanomas. Ki67 is a proliferation-associated nuclear protein expressed during all active phases of the cell cycle and is absent in resting cells.

Intended use

The antibody cocktail labels Melan A and Ki67 in both normal and neoplastic cells and has a cytoplasmic and nuclear staining pattern respectively. Cellular proliferation is correlated with the progression of melanoma. This antibody cocktail is useful for assessing proliferative activity of melanoma when used in a panel of antibodies

Specifications
product code: 8480-C010	Clone: EP43 / GM010 [Rabbit/ Mouse Monoclonal]
Staining pattern: Cytoplasmic staining (Melan A, RED), nuclar staining (Ki67, DAB)	Control tissue: Skin, kidney, tonsil
Associated panels: Dermatopathology
Assessment criteria
Strong, distinct cytoplasmic staining reaction of virtually all melanocytes in skin (Melan A, RED) A weak to moderate reaction in the melanocytic dendrites in most melanocytes (Melan A, RED)
A moderate to strong, distinct nuclear staining reaction in 80-90% of the germinal centre B-cells in both the light and the dark zone and in the vast majority of the suprabasal squamous epithelial cells (Ki67, DAB) No staining reaction must be seen in the vast majority of mantle zone B-cells in tonsil (Ki67, DAB)
No staining in the epithelial cells of tubulus in kidney (Melan A, RED)
Examples: proliferativity of melanoma's