DUO PIN cocktail

Prostatic intraepithelial neoplasia (PIN) is a condition defined by neoplastic growth of epithelial cells within preexisting benign prostatic acini or ducts. PIN is a non-invasive precursor to prostate adenocarcinoma and demonstrates preservation of basal cells. In contrast, prostate adenocarcinoma is characterized by a complete lack of basal cells. The immunohistochemical combination of nuclear p63 expression and cytoplasmic CK5/6 expression aids in detection of peripheral basal cells while cytoplasmic expression of AMACR in glandular cells aid in the detection of 2 potentially neoplastic proliferation.. 

Intended use

This antibody cocktail, which utilizes a brown DAB chromogen for p63 and CK5/6 and the AP Red chromogen for AMACR, is useful for distinguishing benign and malignant proliferations in the prostate, when used in a panel with other antibodies

Specifications
product code: 8485-C010	Clone: 13H4 [Rabbit Monoclonal], MX013, D5/16B4 [Mouse monoclonal]
Staining pattern: Nuclear staining (p63, DAB), cytoplasmic staining (AMACR, RED / CK5/6, DAB)	Control tissue: Kidney, prostate, esophagus, liver, tonsil, placenta
Assessment criteria
A moderate to strong granular cytoplasmic staining of epithelial cells lining renal proximal tubules (AMACR, RED) A weak, granular cytoplasmic staining of epithelial cells of the renal distal tubules (AMACR, RED)
A moderate to strong, distinct nuclear staining reaction in almost all squamous epithelial cells in the tonsil and an at least weak nuclear reaction in scattered lymphocytes in the tonsil (p63, DAB) A moderate to strong, distinct cytoplasmic staining reaction of virtually all squamous epithelial cells of the tonsil (CK5/6, DAB)
At least weak to moderate, distinct nuclear staining reaction of dispersed cytotrophoblastic cells in the placenta (p63, DAB)
An at least weak, predominantly cytoplasmic (but also membranous) staining reaction of scattered columnar epithelial cells of intercalated ducts of pancreas (CK5/6, DAB)
No staining reaction in luminal epithelial cells and smooth muscle cells in prostate (p63, DAB) The epithelial cells of the prostate must be negative or only show a focal staining reaction (AMACR, RED)
No staining of the epithelial cells of the bile ducts of the liver (CK5/6, DAB)
Example: proliferation in prostate