PD-L1

The programmed-death ligand 1 (PD-L1, also known as CD274) is a transmembrane protein constitutionally expressed on a variety of cell types. When PD-L1 binds the checkpoint molecule PD-1, an inhibitory signal is transmitted which reduces the proliferation of T-cells in lymph nodes and thus allows tumor cells to evade attack from T-cells. PD-L1 expression is detected in most human cancers but not in most normal tissues. Specifically in non-small cell lung carcinoma (NSCLC), PD-L1 expression is increased in higher grade tumors.

Intended use

The anti-PD-L1 antibody is a useful aid in assessing PD-L1 protein expression in human NSCLC when used
with a panel of other antibodies. The use of this antibody is limited to TPS (Tumor Proportion Score) in SCLC, it does not score Optimal (and should not be used) for CPS (Combined Positive Score) scoring!

Specifications
product code: 8263-C010	Clone: RM320 [Rabbit]
Staining pattern: Membranous staining	Control tissue: Tonsil
Associated panels: Lung panel, Gastrointestinal
Assessment criteria	no scan available at this time
A moderate to strong predominantly membranous staining reaction in dispersed crypt epithelial cells in tonsil
A weak to moderate, typically punctuated membranous staining reaction of the majority of germinal centre macrophages and scattered interfollicular lymphocytes and macrophages in tonsil
No staining reaction in the vast majority of lymphocytes and normal stratified squamous epithelial cells in tonsil
No optimal control tissue for PD-L1 is found. The staining pattern changes between the different clones, thus difficult to state a general and interchangeable reliable expected staining pattern!