SMAD4

The SMAD family 4 (SMAD4) or deleted in pancreatic cancer 4 (DPC4) is a tumor suppressor gene that is
inactivated in a sub-set of pancreatic adenocarcinomas. Somatic mutations of SMAD4 have been
discovered in pancreatic duct adenocarcinoma (~ 55%). Alterations in the SMAD4 gene also occur in a
variety of cancers, including gastrointestinal track cancers, such as colorectal carcinoma (10-20%).

Intended use

Anti-SMAD4 antibody is a useful aid in differentiating high-grade pancreatic intraepithelial neoplasia and invasive pancreatic adenocarcinoma from benign/reactive pancreatic conditions when used with a panel of other antibodies

Specifications
product code: 8258-C010	Clone: EP618Y [Rabbit monoclonal]
Staining pattern: Nuclear, and some cytoplasmic staining	Control tissue: Tonsil, pancreas
Associated panels: Carcinoma - general panel, Gastrointestinal
Assessment criteria	no scan available at this time
A strong, predominantly nuclear staining reaction of virtually all basal squamous epithelial cells in the surface epithelium of tonsil
An at least moderate, nuclear but also cytoplasmic staining reaction of normal acinar cells of the pancreas
Pancreatic cancer: No staining reaction in the vast majority of neoplastic cells in the pancreatic adenocarcinoma. Normal infiltrating cells must be positive serving as internal pos. control