Tryptase

Tryptase is a trypsin-like neutral serine proteinase stored predominantly in secretory granules of mast cells and basophils, and upon cellular activation is released into the extracellular environment.

Intended use

Tryptase may therefore be used as a marker for mast cells and basophils and an indicator for their activation/degranulation. Anti-tryptase antibody is also a useful tool for identifying reactive mast cell hyperplasia, mast cell leukemia, and mastocytosis, when used with a panel of antibodies.

Specifications
product code: 8372-C010	Clone: EP259 [Rabbit]
Staining pattern: Cytoplasmic staining	Control tissue: Tonsil, Appendix, Colon
Assessment criteria
A moderate to strong cytoplasmic staining of interfollicular mast cells is seen in tonsil.
No staining in lymphocytes in tonsil
A moderate to strong cytoplasmic staining of mast cells is seen in lamina propria in appendix and colon.
*A weak and diffuse staining pattern in the vicinity of labeled cells may be observed