IgG

Immunoglobulin monomers (Igs) are composed of two identical heavy chains and two identical light chains. Variations in the Fc region of the heavy chains distinguishes five different classes of Igs (IgA, IgD, IgE, IgG, and IgM). Each of these classes can be expressed on the B-cell membrane, and more than one heavy chain class can be expressed by the same cell. In normal, healthy serum IgG is the most abundant of the Ig classes. Igs are produced in mature B-cells and plasma cells, and the amount and type of Ig can vary in different stages of B-cell maturation: Igs in the earlier stages of maturation are present in the cytoplasm, whereas surface Igs are more characteristic of mature B-cells in the mantle zone. Plasma cells lose membranous expression of Igs, but accumulate them at a high level in the cytoplasm and can secrete them into the extracellular space. B-cell derived neoplasms, such as B-cell lymphoma, and plasma cell neoplasms often produce only one type of clonal Ig light chain

Intended use

Anti-IgG antibody is useful for classifying B-cell lymphomas and plasma cell neoplasms when used with a panel of other antibodies. The IgG antibody may also be used to help calculate the ratio of IgG4 positive plasma cells to IgG positive plasma cells in tissues from IgG4-related disorders.

Specifications
product code: 8383-C010	Clone: RM116 [Rabbit]
Staining pattern: Membranous staining	Control tissue: Tonsil, lymph node
Associated panels: Gastrointestinal
Assessment criteria
In tonsil and lymph node, a moderate to strong cytoplasmic staining of 60-70% of the plasma cells; a moderate to strong cytoplasmic and membraneous staining of immunoblasts in the germinal center; some background staining in blood vessels, connective tissue and epithelial cells is accepted due to the presence of extracellular IgG.